Composite

Part:BBa_K912031:Design

Designed by: Grant Nicholas   Group: iGEM12_Northwestern   (2012-10-03)


pH Inducible Lysis System(m)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2609
    Illegal NheI site found at 2632
    Illegal NheI site found at 3059
    Illegal NheI site found at 3082
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3416
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2214
    Illegal AgeI site found at 2284
    Illegal AgeI site found at 3631
    Illegal AgeI site found at 4375
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2865


Design Notes

None besides designing primers properly


Source

Lysis:registry CP/RBS: registry clc-ec1: PCR from genome Pgad: PCR from vector from CUHK 2011 team


References